Been there, done that
The question is when you are playing the video, how are you doing that?
-side note: whole long issue regarding copyright to not ignore, as that can get your channel blocked by automated bots
short answer - yes, should be doable, but how exactly depends on info you haven't provided
I'd be inclined to play video as its own source in OBS, and then mute the sound system (soundboard/mixer which is a separate specific source in OBS, right? you aren't using Desktop Audio hopefully) altogether. That way, you have cleanest audio and video as direct from file, via Media Source/VLC Player on OBS PC, to stream.
- Back during lockdown, when intermixing live and pre-recorded House of Worship content, I found the need for Advanced Scene Switcher plugin (often referred to in these forums as AdvSS), to automate a bunch of the transitions (ie had a scene with pre-recorded content, and not the house mixer... so we couldn't make a mistake)
https://obsproject.com/forum/resources/advanced-scene-switcher.395/
- Then I also used the Media Controls Plugin <
https://obsproject.com/forum/resources/media-controls.1032/> so when playing pre-recorded content, I could see a timer for remaining time in video. AdvSS would auto-change scenes when video ended as I configured. I'm not sure if latest OBS Studio UI changes negate the need for this plugin?
One consideration is whether you want background sound (ie congregation singing along) as part of the stream. Obviously, if you mute your mixer/soundboard, you won't pick up audience participation.
Our older analog mixer (soundboard) has an AUX mix, meaning I setup a separate mix for mics for piano, choir, and pipe organ (which do NOT need in-house amplification (sent to front of house speakers). At times I'd like to mute a few mics. The challenge is our mixer is designed for set and forget, and in a small closet; and is older analog.. so I can't remotely adjust individual channels on the mixer (as can be done with some newer mixers). You also have to consider with your worship style, and volunteers, etc, the expertise of those (if any) running livestream and /or mixer. Some worship styles have dedicated sound engineer for every service (praise/band style, and others)... if that is your scenario, you should have one mix for in-house amplification/speakers., and another for livestream audience. And sound engineer should be able to control both, live during service. There are other scenarios. Our setup is no sound engineer, no-one touches mixer unless something is really wrong (which is uncommon... once every 6 months or so??). However, we do have a person running the live stream, changing OBS scenes (intro, welcome, then camera video width (I configured 3 options), advancing Service Bulletin (PowerPoint) to match service, acting as Digital usher, etc. The audio in our case, is the AUX audio mix from our mixer (in stereo). As such, we do NOT have access to individual mic channels in OBS.
MacOS (vs Windows) benefits from a better audio subsystem, and depending on your mixer, mixer config, and connection, you might be able to configure your mics to be visible in OBS. However, as sound people will tell you, for cleaner sound (which is more important than video), it is usually better that handle multi-channel audio outside of OBS (in the mixer itself, or in a DAW (digital audio workstation, ie software mixer). My desire, eventually/maybe, is to get a DAW running on our OBS computer (which has plenty of compute power to run OBS Studio and the DAW at same time), and handle some simple audio mixing in the DAW, then outputting that to OBS Studio. How easy/hard that is, and at what cost, depends on your specific gear (sorry.. it depends)
so - not that my expertise is in sound (it isn't, by a long shot). But telling us exactly which mixer, and how that is connected to OBS (Dante over network, USB, firewire, or ??), and is the mixer configured for separate in-house speaker/amplification vs livestream? will help.
And within OBS Studio, how are your sources setup? posting you OBS Log (per pinned post in this forum) would be helpful
There are a number of us in these forums helping out with House of Worship streaming. hopefully we can get you sorted out quickly (though I normally don't check these forums over the weekend)