There's nothing like best settings. Youtube butchers whatever you upload to it no matter what you do.
Besides, what you do is wrong anyway: recording with OBS is lossy and the bitrate is low, and then you feed it to Youtube.
If you want to upload stuff to Youtube, do a lossless capture with MSI Afterburner using Lagarith codec. It will look bad too, not nowhere near as bad as if you reencode a video twice with lossy compression.