My suspicion is that the husband (condolences on your loss) was regularly adjusting the sound mixer during the service.
What is common in a House of Worship (HoW) environment is 1 set of sound board settings for in-person attendees (often Main Out), vs different settings in an Aux(iliary) Out going to stream (OBS). And if you have a big sound panel, then it gets even more complex than that
So, some HoW are setup assuming an ongoing/constant adjustment to the mixer. That requires a trained person. Lacking such will result in bad sound. And there really isn't an easy button fix for this. And I'm guessing that is original posters setup. Then there are HoW like ours where the mixer is in a closet and rarely gets touched. depends on service style, mic usage (not age/type) and expectations. For our liturgical style environment, with Choir, Pipe Organ, and occasional piano and/or Bell Choir, fixed audio/mixer settings work ok for us. Certain mics are sent only to livestream (not including/amplified) in Main Out to speakers.
I mention this as the answer is ... it depends
Also, most FB users are listening on absolutely the worst speakers possible (phone, tablet, $10 headphones, headset design for spoken voice, etc). And FB (and YouTube and everyone else) compresses audio in livestream, which doesn't help. So, assuming livestream sounded ok before, but now doesn't, I'm betting a setting (or many) on the sound board was changed, and that is the problem. What to change depends on the board, and a lot of factors having nothing to do with the PC or OBS. What may be complicating matters is if someone changed OBS settings trying to overcome a sound board setting, or changed the audio connection setup between sound board and OBS PC (depending on analog or digital connection). In which case, there could be multiple, compounding adjustments required. And realistically, a person is going to need to be onsite troubleshoot.
Assuming in-person mixing is working fine, I'd start with
1. figuring out how audio from mixer is getting to OBS PC. And make sure that is still setup correct (sorry, what that means?... depends on your specific setup)
2. Assuming large mixer board, then I'm assuming sophisticated setup, so separate audio mix for livestream vs in-house amplification (and possibly more for cry room? overflow room? etc). Someone trained/familiar with the sound board will have to figure that out (has nothing to do with OBS, livestream, etc)
3. Once livestream audio path determined, then during rehearsal or test session, adjust this livestream audio mix (Aux or ??) to sound good on the OBS PC. With OBS you can Record and not stream if need be (for when sound board isn't in sound-proof room, so you need to record, then check sound, which is what I have to do cause when Pipe Organ is going, it overwhelms the best headphones ;^)
4. then (sorry, not done yet) as Step 3 provides quality audio to livestream, however, it is not yet optimized for common listening environment (crappy phone speakers)... Be aware that good/optimal sound for home theater setup vs smartphone won't be the same, so you have to pick what to optimize for. In our case, and I suspect most livestreams, will optimize for worst -case scenario (smartphone) and call that good enough for the rest. The setup is not all that dissimilar to radio broadcast audio optimization/settings. A typical setup is to use compression, which reduces dynamic range. Whether that is ok depends on service and music style.
I've not heard of a HoW doing 2 streams, 1 for smartphone/bad speaker users, and another for those wanting high-fidelity audio experience, but it would be possible (I mention this in terms of the 'art of the possible', not a recommendation)