and I'm sure your next question is how?
and that ... depends
depends on your EXACT audio setup (there is a HUGE range of setups out there), the Audio setup within the Operating System, and what exactly you mean by muting audio going to headset... I presume you meant muting 'something specific' vs other audio still coming through to headset?
OBS Studio has an Option to output its audio back to computer (Monitoring on). If [Advanced Audio Properties for Audio source] Monitoring is Off, then Audio will be output to Stream/Recording, but NOT back to Desktop Audio.
These articles are older, but may help you get an understanding - OBS Audio related articles
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